Perubahan Apoptosis Sel Asinar Kelenjar Parotis Akibat Pajanan Radiasi Sinar-X Dosis Rendah (Apoptosis alterations in Parotid Acinar Cells due to a Low Dose X-Ray Irradiation)

Abstract

Background: Radiodiagnosis is one of x-ray used in denti stry. It is important to establish diagnosis and determine treatment plans by using low dose irradiation. Dental x-ray using ionizing radiation can cause ionization on the objects. Patients frequently experienced radiation involved salivary gland, especially parotid gland consisted serous acinar cell s. Parotid acinar cells are very radiosensitive. The irradiation may induce DNA damage and cells apoptosis (cell death). Objective: to investigate effect of low dose x-ray exposure to aci nar cell apoptosis of parotid glands. Methods: This study was in vivo study usi ng animal models. Eighteen male rats (200-250 g) were divided into three groups, control, treatment group irradiated with 1.54 mGy and treatment group irradiated with 7.85 mGy on the parotid area about one second. Parotid tissue samples were collected at the 3 day after irradiation and undertaken histology preparation using Haematoxylin-eosin staini ng. Apoptotic index was examined under light microscope at 400x magnification by two observers. Result: There was significant difference on apoptotic index between control and treatment groups (p<0.05). Conclusion: Low dose x-ray exposure increased parotid acinar cell apoptosis of male wistar rats.