Uji biokimiawi sistem API 20 A mendeteksi Porphyromonas gingivalis isolat klinik dari plak subgingiva pasien periodontitis kronis

Abstract

Background: Porphyromonas gingivalis, a black-pigmented Gram-negative anaerobe, is widely implicated as an important etiological agent in the pathogenesis of periodontal disease. Identification of P. gingivalis clinical isolate will advantage in research efforts aimed at developing oral intervention methods against this bacterial species, and thus for prevention and treatment of periodontal disease. Purpose: This study was to evaluate the ability of API 20A to identify P. gingivalis clinical isolate. Methods: The methods were anaerobic culture and identification of P. gingivalis from bacteria Black-pigmented Gram-negative anaerobes obtained from subgingival plaque of patients with chronic periodontitis, which based on API 20A system, Gram staining, colony and microscopic morphology. Results: API 20A system detected P. gingivalis (P. asaccharolytica) clinical isolate obtained from subgingival plaque with probing depth ≥ 3 mm. Colony and microscopic morphology showed that these bacteria were Gramnegative strict anaerobic coco-bacilli dark brown-black pigmented. They grew in culture media forming convex, smooth glossy colonies of 1-2 mm diameter. There were not differences when P. gingivalis clinical isolate were compared with P. gingivalis ATCC 33277. Conclusion: The API 20A appears to be a promising method for identification of P. gingivalis clinical isolate.