PENGEMBANGAN SENSOR POLIFENOL BERBASIS NATRIUM META PERIODAT DAN 3-METIL-2-BENZOTHIAZOLINON HIDRAZON UNTUK KONTROL KUALITAS PRODUK MINUMAN TEH DALAM KEMASAN

Abstract

Polyphenol sensor based on sodium meta periodate and 3-methyl-2benzothiazolinone

is an analysis device for detecting polyphenol in beverage tea product. The purpose of sensor development is for controling the quality of beverage tea product especially green tea product simply, easily, and efficiently. The polyphenol sensor has been made by immobilized reagent (mixed of 8mM sodium meta periodate (NaIO ) and 24 mM 3-methyl-2-benzothiazolinone hidrazone (MBTH) (1,5:1 v/v)) on whatmann filter paper with a volume of 10 µl. Polyphenol sensor has been characterized and the result is polyphenol sensor has response time about 9 minutes, has linearity at concentration range 25-300 ppm, with correlation coefficient (r) =0,999. This polyphenol sensor has sensitivity about 0,1566 per ppm, while LOD = 10,199 ppm CE and LOQ = 33,997 ppm CE. Interference or selectivity of the polyphenol sensor by Vitamin C produce interference less than 5% untill 1000 ppm of vitamin C (1500 µg). Sensor precision parameter comply with RSD < 2% which exactly 0,628% and the accuracy parameter of polyphenol sensor has a value of recovery about 96,784%. This polyphenol sensor has been applied to some beverage green tea product and this methode give results that significantly indifference with spectrophotometry UV-Vis