Optimization of Centrifugation Speed and pH in Extraction of Uricase Enzyme from Goat Liver
Date
2022-12-21Author
HANDAYANI, Wuryanti
UTARTI, Esti
KRISMIADI, Riki Juni
RATNADEWI, AA. Istri
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The human body does not have an enzyme that can
break down uric acid, so the accumulation of uric acid can cause
disease. This problem can be overcome by uricolytic therapy by
utilizing the activity of the uricase enzyme. In this study, the
uricase enzyme was extracted from goat liver by optimizing the
centrifugation speed and the extraction pH. The purpose of the
optimization is to get maximum uricase activity. Uricase
extraction to optimize centrifugation speed using borate buffer
pH 8.5 then centrifuged at 7.000; 9.000; 11.000; 13.000 and
15.000 rpm with a temperature of 4oC. Furthermore, pH
optimization was carried out using pH buffers 6, 7, 8, 9, 10 and
11 by centrifuging the optimum speed obtained. The crude
extract obtained was further tested for its enzyme activity. The
results showed that the highest uricase activity was achieved if
the extraction was carried out at pH 8 using centrifugation at an
optimum speed of 13.000 rpm. The higher uricase activity
indicates that the extracted uricase concentration is increasing.
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