Revealing of free radical scavenging and angiotensin I-converting enzyme inhibitor potency of pigmented rice seed protein
Date
2020-12-01Author
SUSILOWATI, Erlin
SANJAYA, Bella Rhea Lavifa
NUGRAHA, Ari Satia
UBAIDILLAH, Mohammad
SISWOYO, Tri Agus
Metadata
Show full item recordAbstract
The potential source of bioactive protein from pigmented rice seed was investigated by buffered extracting from nine varieties
(Merah-SP, Gogo Niti-2, Merah Wangi, Super Manggis, Lamongan-1, Mota, Ketan Hitam-2, Beureum Taleus, and Aek Sibundong)
and non-pigmented rice (IR-64) as a control. The potent contributor to free radical scavenging of extracted proteins was
evaluated by analyzing their free amino acid composition. The free radical scavenging and angiotensin-I converting enzyme
(ACE-I) inhibitory activity of these proteins were analyzed in-vitro. The free radical scavenging activity was analyzed using
various standard methods, including radical cation 2,2-azinobis-3-ethylbenzothiazoline-6-sulphonate acid (ABTS•+), hydroxyl
radical (OH•
) and defense against radical-mediated DNA damages by hydroxyl. Total free amino acid content ranged between
77.7-181.2 g/100 g protein, and among them, Ketan Hitam-2 displayed the highest abundant antioxidant amino acid grouping
(23.23%) and shown significantly higher ABTS•+ activity (IC50=8.64 µg/mL) and OH•
activity (IC50=20.33 µg/mL). Moreover,
Ketam Hitam-2 also exhibited notable ACE-I inhibitory activity (IC50=6.20 µg/mL) and protected hydroxyl-induced oxidative
damage to DNA. The in-vitro systems for free radical scavenging and ACE-I inhibitory were used to acquire the data. The
potency of Ketan Hitam-2 seed protein could be utilized as a natural nutraceuticals compound
Collections
- LSP-Jurnal Ilmiah Dosen [7287]