In-vitro Antioxidant Capacities and Genetic Classification of Indonesian Selected Pigmented Rice

Abstract

Rice is a world-famous cereal food divided into pigmented and non-pigmented rice. Pigmented rice is popular as healthier food than non-pigmented rice due to its potency as an antioxidant. Nevertheless, the potential of pigmented rice has not been wide ly studied. Indonesian selected pigmented rice protein’s antioxi dant potential and the non-protein compound were in-vitro stud ied. The antioxidant potencies were evaluated by extracting fresh seeds of nine pigmented rice (Aek Sibundong, Beureum Taleus, Gogo Niti-2, Lamongan-1, Merah SP, Merah Wangi, Mota, Ketan Hitam-2, and Super Manggis) and non-pigmented rice (IR-64) as control. Various free radical scavenging methods to determine the antioxidant activity (ABTS•+, DPPH•, OH• and O2-) were conducted. Meanwhile, the genetic classification was performed by a simple sequence repeat (SSR) marker to determine the relationship between varieties. The results showed that protein of Ketan Hitam-2 had the highest ABTS•+ radical scavenging (98.06%), followed by Beureum Taleus (42.54%). Ketan Hitam-2 protein also showed the highest OH• and O2- activities (43.49% and 6.02%, respectively). The highest DPPH• potency of the non-protein compounds also shown by Ketan Hitam-2 (32.23%) with the activity of OH• and O2- (20.63% and 14.56%, respec tively). These results showed that Ketan Hitam-2 has the highest potency as an antioxidant, which could be recommended as a nutraceuticals compound