Low Oxygen Tension Inhibits Senescence in Bone Marrow Mesenchymal Stem Cells (BMSCs)
Abstract
Stem cells have been used in regenerative medicine but are so few in the body that they
require cell culture. Stem cell culture was performed under normal oxygen tension, and
passage was carried out until the number of cells was sufficient for therapy. Stem cell
cultures under normal oxygen tension do not match the stem cell microenvironment,
leading to premature senescence. This study aims to determine the association of low
oxygen tension with premature senescence of mesenchymal stem cells (MSCs) through
inhibition of p21 expression by HIF-1. The research method used rabbit bone marrow in
New Zealand as a source of MSCs. The results of isolation of MSCs were divided into two
groups cultured on normal and low oxygen tension until 10 passages. Cells were identified
using flow cytometry cd105 and cd34. At early and late passage, the expression of p21
and HIF-1 were examined using immunofluorescence while senescence was examined
using β-galactosidase assay. The results showed that in low oxygen cultures HIF-1
expression increased significantly (p <0.05) while p21 expression decreased significantly
(p <0.05) as did the β-galactosidase assay. The conclusion of this research is low oxygen
tension culture able to decrease premature senescence culture of invitro stem cells
mesenchymal through obstacles p21 by HIF-1.
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- LSP-Jurnal Ilmiah Dosen [7292]