Mycorrhizal fungi Glomus spp. propagation in zeolite enriched with mycorrhiza helper bacteria for controlling nematode in coffee
Date
2021-10-01Author
ASYIAH, Iis Nur
HINDERSAH, Reginawanti
HARNI R, Harni
FITRIATIN B N, Fitriatin
ANGGRAENI, Wulan
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Arbuscular mycorrhizal fungi (AMF) play a role in suppressing the nematode
Pratylenchus coffeae. Mycorrhizal helper bacteria (MHB) can increase the effectiveness of AMF
to control the diseases. The experimental purpose was to increase the spore population of AMF
Glomus spp. in zeolite-based formulation inoculated with liquid consortia of Pseudomonas
diminuta and Bacillus subtilis as MHB. The experimental design was a completely random
design with six treatments consisted of 106
, 107
, 108, and 109 CFU/mL MHB liquid inoculants.
The control treatments were water and 2% molasses. All treatments were replicated four times.
A total of 300 mL/pot Liquid inoculant of MHB have been inoculated a three day before
transplanting the maize seedling to the Zeolite inoculated with Glomusspp. in the pot. One month
after MHB inoculation, Glomus formulation in Zeolite with different levels of MHB increased
the degree of infection. Three months after MHB inoculation, spore content in Zeolite increased.
The density of P. diminuta and B. subtilis in zeolite-based mycorrhizal inoculant increased at the
end of the experiment. Liquid inoculant MHB contained 108 CFU/mL enhanced spora number
fourth times compared to the control. This experiment suggests that P. diminuta and B. subtilis
were effective to increase the spore density of AMF inoculant.
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- LSP-Jurnal Ilmiah Dosen [7292]