PENGEMBANGAN DAN VALIDASI METODE KLT DENSITOMETRI UNTUK PENETAPAN KADAR INULIN DALAM EKSTRAK AIR UMBI BENGKUANG

Abstract

ABSTRACT A simple, sensitive, selective and specific, precise and accurate TLC densitometry method has been developed and validated for determination of inulin in water extracts of yam bean as the stationary phase and using glacial acetic acid :methanol p.a: aquabidest viii 254 C for 10 min, then stained with a mixture of aniline: diphenylamine: phosphoric acid

C for 15 minutes. Quantitative evaluation was performed by measuring the absorbance of the analyte spot at 380 nm. The linear regression data for the calibration plots of inulin showed good linear relationship with r = 0,99610 in the concentration range 6002400 ppm. The

minimum detectable amounts were found to be 35,515 ppm and the limits of quantitation were found to be 118,383 ppm. Repeatability precision, as RSD was 2,754% and Intermediet precision, as RSD was 1,935%. Accuracy of the method was determined through recovery studies by adding known quantities of standard drug to the pre analyzed test solution and was found to be 100,161% ± 1,839%. This TLC Densitometry method is selective and specific, precise, and accurate and can be used for routine analysis of inulin.