| dc.description.abstract | Wound healing is a physiological process that occurs progressively through
overlapping phases. Tissue oxygenation is an important part of the complex regulation for
wound healing. Hyperbaric Oxygen (HBO) therapy is a method of increasing oxygen delivery
to tissues. The therapy improves tissue oxygenation and stimulates the formation of H2O2 as a
secondary messenger for Tumour Necrosis Factor alpha (TNF α), e-NOS, VEGF and Nuclear
Factor Kappa Beta phosphorylation (NF-Kb) which play an important role in the rapid
transcription of a wide variety of genes in response to extracellular stimuli. This study aims to
determine the effects of Hyperbaric Oxygen therapy in enhancing the expressions of e-NOS,
TNF-α, VEGF and wound healing. This study is an animal study with a ‘randomized control
group of pre-test and post test design’ on 28 Wistar rats. Randomly, the rats were divided into
4 groups with 7 rats in each group. The HBO treatment group 1 received 5 sessions of HBO
2.4 ATA in 3x30 minutes; the HBO treatment group 2 received 10 sessions of HBO 2.4 ATA
in 3x30 minutes; and each of the control groups were without HBO. Each of the 28 male rats
were given a full thickness excisional wound of 1x1cm. Examinations of e-NOS, TNF-α,
VEGF expressions and wound healing were performed on day-0 (pre-HBO) and day-5 HBO or
on day-0 (pre-HBO) and day-10 HBO.The resultsshowthat the Hyperbaric Oxygen therapy can
improve e-NOS (p=0.02), TNF-α (p= 0.02), VEGF expression (p=0.02) and wound healing
(p=0.002) significantly in the provision of HBO 2.4 ATA for 3x30 minutes in 5 sessions over 5
consecutive days. While the 10 sessions of HBO 2.4 ATA for 3x30 minutes over 10
consecutive days only increase e-NOS (p=0.02), TNF-α (p=0.04), VEGF expression
significantly (p=0.03) but do not improve wound healing significantly (p=0.3) compared with
no HBO. The study concludes that HBO can improve the expressions of e-NOS, TNF-α,
VEGF and wound healing in the provision of HBO 2.4 ATA for 3x30 minutes in 5 sessions,
while the 10 sessions of HBO 2.4 ATA for 3x30 minutes only increase e-NOS, TNF-α, VEGF
expression but do not improve wound healing. | en_US |
