Overexpression of Sucrose Phosphate Synthase Enhanced Sucrose Content and Biomass Production in Transgenic Sugarcane
Date
2020-02-06Author
ANUR, Risky Mulana
MUFITHAH, Nurul
SAWITRI, Widhi Dyah
SAKAKIBARA, Hitoshi
SUGIHARTO, Bambang
Metadata
Show full item recordAbstract
Sucrose phosphate synthase (SPS) is a key enzyme in sucrose synthesis, which
controls sucrose content in plants. This study was designed to examine the e cacy of the
overexpression of SoSPS1 gene on sucrose accumulation and carbon partitioning in transgenic
sugarcane. The overexpression of SoSPS1 gene increased SPS activity and sucrose content in
transgenic sugarcane leaves. More importantly, the overexpression enhanced soluble acid invertase
(SAI) activity concomitant with the increase of glucose and fructose levels in the leaves, whereas
sucrose synthase activity exhibited almost no change. In the stalk, a similar correlation was observed,
but a higher correlation was noted between SPS activity and sugar content. These results suggest that
SPS overexpression has both direct and indirect e ects on sugar concentration and SAI activity in
sugarcane. In addition, SPS overexpression resulted in a significant increase in plant height and stalk
number in some transgenic lines compared to those in non-transgenic control. Taken together, these
results strongly suggest that enhancing SPS activity is a useful strategy for improving sugarcane yield.
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- LSP-Jurnal Ilmiah Dosen [7287]