Aquoeous Extract of Neem Leaves (Azadirachta Indica) Decrease Expression of Immunoglbulin E (IgE) and Interleukin 4 (IL-4) in Gingiva Tissue of BALB/c Mice Injected by Ovalbumine
Date
2017-11-29Author
DEWANTI, I Dewa Ayu Ratna
SUSILAWATI, I Dewa Ayu
LESTARI, Pujiana Endah
BUDIRAHARDJO, Roedy
WULANDARI, Erawati
YANI, Ristya Widi Endah
WIBISONO, Sunlip
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Show full item recordAbstract
Allergy has been such a difficult disease to cure. Some anti-allergic drugs
on the market caused side effects such as dryness of mucous membranes, confusion,
dizziness, dry mouth, constipation, difficulty urinating and blurred vision, bull neck
and moon face. It is difficult to find anti-allergic drugs with minimum side effects. Aim.
The research aimed to find alternative anti-alergic agent, it was aquoeous extract of
Neem leaves. In particular, this study analysed modulation of aquoeous extract of
Neem leaves to expression of Ig E and IL-4 in gingival tissue. Materials and Methods.
A total 15 BALB/c Mice was divided into three groups, i.e controle group (no treatment
were introduced), Ova group (On days 0, 7, 14 submucous injected with ovalbumine),
Neem group (were given with aquoeous extract of Neem leaves 200 mg/day/kg
body wieght and injected with ova). Ova injection was 25 μL (in 2 mg/mL PBS), while
neem leaves extract were given by oral sondation. IgE and IL-4 were analysed in
gingival tissue on day 21 by imunohistochemistry method. Parameters are amouts
of leucocytes that expressed IgE and IL-4. Results. ANOVA and LSD test showed that
aquoeous extract of Neem leaves could decrease IgE and IL-4 expression significantly
(p<0,05) in gingival tissue. Conclusion. Aquoeous extract of Neem leaves could be
used as an alternative for anti-alergic agent.
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- LSP-Jurnal Ilmiah Dosen [7301]