| dc.contributor.author | Avivi, Sholeh | |
| dc.contributor.author | Dietzgen, Ralf G. | |
| dc.contributor.author | Higgins, Colleen M. | |
| dc.contributor.author | Sudarsono, Sudarsono | |
| dc.date.accessioned | 2019-08-21T03:01:11Z | |
| dc.date.available | 2019-08-21T03:01:11Z | |
| dc.date.issued | 2019-08-21 | |
| dc.identifier.uri | http://repository.unej.ac.id/handle/123456789/91998 | |
| dc.description | Indonesian Protein Society, Proceeding of International Seminar and Workshop on Protein
Jember, October 29-30, 2014 | en_US |
| dc.description.abstract | The aims of this research were to (1) compare the effectiveness of established Agro bacterium
mediated transformation and bombardment mediated transformation on peanut (2) evaluate the
integration of green fluorescent protein (GFP) and hygromycin resistance (hyg
r
) gene on the
peanut embryosomatics. To achieve those objectives we shoot 1200 explants using
bombardment and transform 1000 explants using Agrobacterium mediated transformation. Both
of Agrobacterium mediated transformation and particle bombardment could give positive
embryos transformed. Particle bombardment gave 4.5% transformation efficiency while
Agrobacterium mediated transformation gave only 1.8%. From this research, we concluded that
particle bombardment more efficient and gave more transgenic explants than Agrobacterium
mediated transformation. | en_US |
| dc.language.iso | en | en_US |
| dc.subject | Agrobacterium | en_US |
| dc.subject | bombardment | en_US |
| dc.subject | GFP | en_US |
| dc.title | The Efficiency of GFP Gene Transformation on Peanut Embryo somatic Using Agrobacterium and Particle Bombardment | en_US |
| dc.type | Prosiding | en_US |
