| dc.description.abstract | Self-Nanoemulsifying Drug Delivery System (SNEDDS) has
potential to be developed for oral protein delivery because it is
free from water, hence preserving the stability of protein,
protecting protein from enzymatic degradation, and enhancing
the protein permeability in the gastrointestinal tract (GIT).
However, protein-based SNEDDS formulation is challenging due
to low solubility property of protein in oil, which is towards zero.
This present study aimed to obtain the most compatible SNEDDS
for protein using HLB approach. Bovine serum albumin (BSA)
was used as a protein model. A number of 78 formulas with HLB
ranging between 11 and 15 were screened to acquire stable
SNEDDS composition without the presence of phase separation.
Of 13 stable formulas, two were selected (F30 and F45) with HLB
15, and then loaded with BSA. Physical characteristics of both
formulas were then evaluated and these results suggested that
SNEDDS with single hydrophilic surfactant (F45) and HLB 15 was
the best formula for protein template as the stability testing
showed that phase separation and precipitation did not appear. It
was robust to pH and dilution with percentage of transmittance
of 96.40±1.05% and the droplet size of 180.9nm. F45 had also
uniform distribution of droplets size since the polydispersity index
was less than 0.1. The zeta potential of F45 was -0.12mv with
loading efficiency 83.57±1.77%. The emulsifying time of F45 was
> 2min due to the formation of crystalline gel that was difficult to
disperse. | en_US |
