| dc.description.abstract | Particulate delivery system can be used for improving the
efficacy of protein and peptide drug. In addition to a polymerbased
particulate delivery system, self-nanoemulsifying drug
delivery system (SNEDDS), a lipid-based delivery system, is
currently developed for either less water-soluble or soluble drugs.
This study aims to design SNEDDS for oral insulin administration
and its in vitro-in vivo study. The SNEDDS template was designed
using D-optimal mixture design and was analyzed using software
Design Expert 7.1.5. The obtained optimum template was loaded
with insulin and evaluated for its transmittance percentage,
emulsification time, particle size, zeta potential, stability, the
amount of insulin in vitro diffused across rat intestine, and insulin
serum concentration after oral administration. The study results
revealed that the optimum template of SNEDDS formula consisted
of 10% (w/w) Miglyol 812N, 65% (w/w) Tween 80, and 25% (w/w)
propylene glycol. These optimum template then was loaded with
insulin and characterized. SNEDDS insulin has particle size of
12.0±1.7 nm, zeta potential of +0.16mV, transmittance of >90%,
and emulsification time of < 60 seconds. The stability study
showed that SNEDDS insulin was stable from both precipitation
and phase separation. The amount of insulin transported from
SNEDDS formula in vitro was 32.45±2.03% and non-SNEDDS
formula was 10.44±5.04%. In vivo study of SNEDDS insulin
produced a significantly increased Cmax, AUC, and F value than
insulin non SNEDDS (p < 0.05). In brief, SNEDDS formulation in
this study is a promising approach to increase the effectiveness of
oral insulin. Insulin is better given orally in SNEDDS formulation
than in non SNEDDS formulation. | en_US |
