| dc.description.abstract | There is adipocytokine dysregulation on obesity such as amplified leptin, and reduced adiponectin at serum
levels . Leptin has been proved to increase oxidative stress in endothelial cell. The adipocyte functions as an
important secretory organ via nuclear factorκ
B
(NF-κB)
releasing
a number
of bioactive
molecules
such
as
leptin.
Lycopene,
an
antioxidant,
is
presumed
having
the
ability
to
block
the
atherogenesis
mechanism,
which
is
stimulated
a
proinflamatory
cytokine
and
adhesion
molecules
ICAM-1
by
NF-κB.
Therefore,
the
aim
of
this
research
was
to
prove and
to
determine
whether
lycopene
could
decrease
the
NF-κB
and
ICAM-1
expression
in
Human
Umbillical
Vein
Endothelial
Cells
(HUVECs)
culture
induced
by
500 ng/mL
leptin.
In
vitro
study
used
primary
culture
of
the
HUVECs
were
devided
in
to
7
groups, there
were
(1)
0
ng/mL
leptin
and
0
μM
lycopene,
(2)
induced
by
500 ng/mL
leptin
for
12 hours,
(3)
induced
by
leptin
and
lycopene
with
concentration
10;
25;
40;
55 and 75 μM for 12 hours. Then the identification of NF-κB was applied by using imunocytochemistry
compared with ELISA procedure on cell endothel culture lysate and ICAM-1 expression was measured by
using RT PCR. It was showed that lycopene 25 μM decreased NF-κB level and ICAM-1 expression
significantly in Human Umbillical Vein Endothelial Cells (HUVECs) culture induced by leptin 500
ng/mL.Leptin was increased NF-κB and ICAM-1 expression in HUVECs culture and was decreased by
lycopene. Optimum dose of lycopene is 25 μM. | en_US |
