| dc.description.abstract | The purpose of this study was to determine the
effect of different concentration and time sterilization on
the number of colony-forming units of isolated endophytic
bacteria from Cosmos caudatus kunth. leaf. The
sterilizing agents were used is sodium hypochlorite
(NaOCl). The research consisted of four treatments P1
(NaOCl 5.35%; 3 minutes), P2 (NaOCl 5.35%; 1,5
minutes), P3 (NaOCl 3%; 3 minutes), and P4 (NaOCl
3%; 1,5 minutes). Every treatments have one control
medium and three repetition. The control medium treated
by make streak using C. caudatus leaf that had sterilized
and the three repetition treated by inoculating 100 µL
suspension of C. caudatus leaf with 10
-1
serial dilution.
Result on the control medium is sterile and indicated that
the bacteria in treatments medium is endophytic bacteria.
The CFU in treatments P1 (0x10
2
CFU/ml), P2 (2,9 X 10
CFU/ml), P3 (2,9 X 10
3
CFU/ml), and P4 (2,8 X 10
CFU/ml). The conclusion from this study is there was no
significantly different of different concentration and time
sterilization on the number of colony-forming units of
isolated endophytic bacteria from Cosmos caudatus
kunth. Leaf. | en_US |
