dc.description.abstract | Escherichia coli contributes to the contamination of horticultural products,the prevention of that
bacterial contamination can be carried out using bacteriophage. This research is aimed to determine the
characteristics of bacteriophages and their interactions with host bacteria. Characteristics of isolated
Escherichia coli, morphology of bacteriophages capable of Escherichia coli infection and host range of that
particular bacteriophage were observed. The result revealed that all the bacteriophages obtained namely
IGKR1a, IGKR1b, IGKR2, IGPT1a, IGPT1b and IGMJ1b belong to the group of Myoviridae. Isolated
Escherichia coli namely KR, KP, MJ, PR and PT were used as host bacteria. The host range test showed that
all bacteriophages could infect KR, KP and MJ bacteria. Bacteriophage characteristics can be seen from the
protein profile. The six bacteriophages had similar protein profile and bands size. The thick bands formed
on SDS gel showed the bands of the major proteins and the thin ones were minor proteins. | en_US |