dc.description.abstract | The main objective of this research was to identify quantitative trait loci associated with rice qualities
to provide
reliable
information
for
marker-assisted
selection
and
development
of
new
varieties.
In
total,
120
doubled
haploid
(DH)
lines
developed
by
another
culture
from
the
F
hybrid of a
cross between “Cheongcheong”, a Tongil variety, and “Nagdong”, a japonica variety, were used. A
microsatellite linkage map of 222 markers spanned 2082.4 centimorgans (cM) and covered 12
rice chromosomes with an average interval of 9.4cM between markers. Eight quantitative trait loci
(QTLs) were associated with rice quality, consisting of two QTLs on chromosomes 1 and 9 for
amylose content; three QTLs on chromosomes 8, 9, and 10 for protein content; and three QTLs on
chromosomes 2, 3, and 6 for lipid content. PCR expression levels measured using the SSR markers
RM23914 for proteins and RM6266 for lipids, and RM586 showed a higher degree of amplification.
The present study should be useful for improving the nutritional quality of rice by means of
marker-assisted selection. | en_US |