Pengaruh Pemberian Gel Ekstrak BijiKopi Robusta (Coffea robusta) Terhadap Ketebalan Epitel Gingiva Model Tikus Periodontitis yang Diinduksi Porphyromonas gingivalis

Abstract

Background: Periodontitis is an inflammatory disease affecting tooth supporting tissues. This disease is caused by Porphyromonasgingivalis and can extend into deeper tissues involving alveolar bone. Inflammation is a host defense response that can lead to gingival epithelium destruction. Robusta coffee seed contain caffeine and chologenic acid which can act as anti-inflammation and antioxidant agents. Purpose: The aim of this research was to analyze the effect of robusta coffee seed extract gel administration on the density of gingival epithelium in P.gingivalis induced periodontitis rat models. Methods: Thirty-two samples of rats were divided into 8 groups; negative control group (normal), positive control group (periodontitis + placebo gel), 25% treatment group (periodontitis + 25% robusta coffee seed extract gel), 50% treatment group (periodontitis + 50% robusta coffee seed extract gel) in which each of them was further divided into day 1 euthanasia group and day 7 euthanasia group. Each group consisted of 4 samples. After that HE staining was carried out for epithelium density was measured using software imageraster. Results: K4 (50% robusta coffee seed extract gel group) has higher mean of epithelium density than those of group 2 and 3. Conclusion: The administration of robusta coffee seed extract gel increased the gingival epithelium density of periodontitis rat model.