| dc.description.abstract | Background: Neem leaf is used to increase body immune response, antivirus,
antifungal, antibacterial, and antioxidant. These biology activities are suspected because
of protein in neem leaf. Protein in plants can be used as pharmacogenetic identification
because some proteins in plants posse characteristic can defense to some pathogen
microorganism. The way to know protein in the organism is using profile protein analytic
through SDS-PAGE method. SDS-PAGE separates protein molecules by size, particular
shape and also can determine protein molecular weight. Protein profiles can be obtained
with protein extracts using solvents. Protein is a polar compound which is bonded to a
polar solvent therefore chosen ethanol and aquadest as a liquid as extraction solvent.
Purpose: To know the protein profile of aquadest and ethanol extracts of neem leaf by
using SDS-PAGE. Method: This study used neem leaf as a sample, aquadest and 70%
ethanol as the extraction solvent then analyzed using SDS-PAGE. Result: The results showed
9 protein bands on the aquadest extract of neem leaf and 8 of protein bands on the
ethanol extract of neem leaf. Conclusion: From the study, there was a difference between
the amounts of protein bands aquadest extracts of neem leaf with ethanol extract of
neem leaf. | en_US |
