| dc.description.abstract | simple and rapid high-performance thin-layer chromatography
(HPTLC) densitometric method has been developed for the determination
of mebhydrolin
napadisylate in tablets. After
extraction of
the analyte with a mixture of methanol and NH
OH 25% (100:1.5,
v/v), the extracts were spotted on precoated HPTLC silica gel F254
plates, which were developed with a mixture of methanol and ethyl
acetate (1:1, v/v). Quantitative evaluation was performed by measuring
the absorbance reflectance
of the analyte spots at 287
nm.
The method was validated for specificity, linearity, accuracy, precision,
and robustness.
Good linearity was achieved in the concentration
range 600–1600
ng/spot. The RSD of repeatability and intermediate
precision
were
found to be less than 2%, whereas
the mean of
the
recovery
data was 99.3–100.8%. The
detection limit and quantification
limit were
18.5 and 55.5
ng/spot, respectively. The drug
was subjected to acidic and alkaline hydrolysis, oxidation, dry heat,
and UV treatment. The peak of mebhydrolin napadisylate was not
interfered by those of possible degradation products. | en_US |
