| dc.description.abstract | Rhodamine B is one of the synthetic dyes that forbidden in cosmetics. The study was
conducted to validate TLC-densitometry method for determination Rhodamin B in lipstick at
Jember University area. Samples was taken by purposive sampling method. The analyte were
dissolved with ethanol 70% and cromatographed on silica Gel GF 254 TLC plate using etil
asetat : metanol : amoniak in the ratio (15:3:3) (v/v/v). Then, validation and determination
sample can be done using TLC-densitometry with maximum wavelength 554 nm. Method was
found linear over the concentration range of 6 – 32 ng with correlation coefficient of 0,9965.
Spesificity showed calculation of purity and identity more than 0.99. The limits of detection and
quantitation limits obtained 0.558 and 1.86 ng/spot. The precision test which includes precision
repeatability and intermediate precision, amounting to RSD 6.57% and test accuracy , resulting
% recovery and RSD value of 97.74 % ± 2.54% . Based on these results lipstick sample
purposively sampled none containing rhodamine B and safe consumer use. | en_US |
