| dc.description.abstract | ‘Biduri’ plant is a wild bush in tropical countries which is one of potential protease
source. However, protease extracted from leaf and stamp top of biduri is still green in color
due to contain a protein bounding-chlorophyll. It would be problem if it is used for some food.
The research objective is to study a dechloroplyllation technique in order to obtain protease
with low chlorophyll content but high specific activity. The results showed that the best
dechlorophyllation method of biduri protease could be eluted by celite absorbance. The first
step elution was obtained 16 ml filtrate of biduri protease with low chlorophyll. Consist to the
result above also decreased protein content, with loading capacity was 1.067 gram
biduri/gram celite or 0.015 g chlorophyll/gram celite. However in the second step elution,
after biduri filtrate has been freezed for 12 hours was obtained the dechlorophylated biduri
protease was higher in loading volume. Resulting in increased of loading capacity to be 2.13
gram biduri/gram celite or 0.004 g chlorophyll/gram celite. The chlorophyll decreased to
about < 44 % of chlorophyll from the first step elution, even the specific activity increased
286% compared with the first step elution. | en_US |
