dc.description.abstract | Dengue Haemorraghic Fever (DHF) is an acute Flavivirus infection transmitted by
several species of Aedes mosquitoes with Aedes aegypti as the main vector. During
blood feeding, arthropod vectors inject saliva into vertebrate hosts. The saliva is
biochemically complex, pharmacologically active and plays an important role in
pathogen transmission. The objective of this research is to identify immunogenic
salivary gland proteins of Ae. aegypti against human blood sera of people living in
endemic area. Protein profile from Salivary Glands (SG) of Ae. aegypti was observed
by 12% SDS-PAGE from lab. scale cultures and from landing populations.
Identification of immunogenic proteins from both sample was carried out by using
Western Blot Analysis after cross reaction of Salivary Gland Extract (SGE) with 3
different human sera: from DHF patients, healthy persons who were exposed to Ae.
aegypti and healthy person who were likely not exposed. Sera from healthy people from
non endemic areas and from infants were used as negative controls. Over all, the
protein profiles from lab. scale cultures SGE and landing populations were quite
similar. We found 13 protein bands were identified ranging from 26 kDa up to 255
kDa. We predicted that 255, 56, 31, 27 and 26 kDa are target protein, which is two of
immunogenic proteins were able to cross-react with human sera from people living in
endemic area on 31 and 56 kDa. These bands appeared only in samples from humans
who were previously exposed to mosquitoes bites, and not in humans who had not been
exposed. These immunogenic salivary proteins may serve as human immune response
against Ae. aegypti bites. This result indicated that may the 31 and 56kDa protein has
function as transmitted pathogens. | en_US |