| dc.description.abstract | Dengue Haemorraghic Fever (DHF) is an acute Flavivirus infection transmitted by several
species of Aedes mosquitoes, e.g. Aedes aegypti. During blood feeding, arthropod vectors
inject saliva into vertebrate hosts. The saliva is biochemically complex and
pharmacologically active, and may play an important role in pathogen transmission. The aim
of this research is to distinguish immune response from healthy people in endemic and nonendemic
region, and dengue patients as well. To examine whether mosquito saliva could
elicit humoral immune response in humans under natural conditions, we have collected
plasma from dengue patients, healthy villagers, and people from a non-dengue region around
Jember (East Java). This study was a part of the project approved by the Ethical Committee
of Medical Faculty of Jember University for the human subject protocol. All participants
gave written, informed consent before entering the study. SDS-PAGE analysis was
performed to profile the pro tein contents of the salivary glands from Ae. aegypti, which
revealed a number of proteins with molecular weight ranged from ~28 to ≥142 kDa. To
identify which proteins of the salivary glands are immunogenic in eliciting antibody
responses in humans, we analyzed the salivary gland extracts of female Ae. aegypti by
Western Blot with human plasma. Here we have demonstrated that anti-dengue salivary
protein antibodies occurred predominantly in healthy people from endemic region which
revealed a spesific protein with molecular weight ~ 37 kDa, whereas people from a nondengue
region had no such antibodies. These immunogenic salivary protein may serve well as
an indicator of immune respone in human. Furthermore, these protein may be excellent
candidates for the development of dengue vaccine. Therefore, the extend molecular activity
and identification of ~ 37 kDa protein should be further investigated. | en_US |
