| dc.description.abstract | used as antimicrobial properties. Several researchers have indicated that these properties were
included in polyphenol compounds of coffee. This study aims to analyse polyphenol extracts of
robusta coffee beans effect the damaging of S. mutans cell wall. Methods. This research is a
laboratory experimental research by using the post test only control group design. The variables
that will be observed are the damaging of S. mutans cell wall which are divided into two groups.
Those are treatment and control. The treatment consists of four tubes containing polyphenol
extracts of robusta coffee beans concentration of 100%, 50%, 25%, and 12,5%, whereas control is
only contained of S. mutans. After incubating for about 370 C for 24 hours, the fifth tube was
prepared to observe by using Scanning Electron Microscope (SEM), the method according to the
reference in the Electron Microscope Unit and Integrated Laboratory School of Medicine,
University of Airlangga. Damaging S. mutans cell wall were observed by morphology alteration
such in size alteration, kern formation (blebs), and ghost cell formation. Results. The results of the
research indicated to Mann-Whitney test for damaging S. mutans cell wall such in size alteration,
kern formation (blebs), and ghost cell formation refers that polyphenol extracts of robusta coffee
beans can effect the size alteration, kern formation (blebs), and ghost cell formation to the S.
mutans cell wall significantly (p ≤ 0,05). Conclusion and Suggestion. The polyphenol extracts of
robusta coffee beans effect the damaging of S. mutans cell wall such in morphology alteration
such in size alteration, kern formation (blebs), and ghost cell formation. Further research is
needed on the ability of other compounds from the extracts of robusta coffee beans in damaging
the bacterial of cell wall. | en_US |
