Show simple item record

dc.contributor.authorF r e n g k y Her m awan Had i P r a s e t yo
dc.date.accessioned2014-01-22T23:51:49Z
dc.date.available2014-01-22T23:51:49Z
dc.date.issued2014-01-22
dc.identifier.nimNIM081510501149
dc.identifier.urihttp://repository.unej.ac.id/handle/123456789/21469
dc.description.abstractP e r t um b u ha n da n pe r ke m ba n ga n t a n a m a n d i t e n t uka n o l e h l a j u f o to s i n t e s i s da n h a s il a k hi r da r i f o t o s i n t e s i s a da l a h s ukr o s a da n pa t i . S e l a nj ut ny a h a s il f o t o a s i mi l a t y a n g b e r upa s ukr o s a ke m ud i a n d i t r a ns l o ka s i ka n ke s e l ur u h b a g i a n t a n a m a n u n t uk pe r t um b u h a n da n pe r ke m ba n ga n t a n a m a n . P r o s e s m e t a b o l i s me ka r b o hi dr a t m e nj a d i s ukr o s a t e r s e b ut m e li ba t ka n pe r a n UD P g l u c o s e da n s u c r o s e ph o s pha t e s y n t h a s e ( S P S ) , s e da n gka n t r a n s l o ka s i s ukr o s a da r i s our c e ke s in k d i ke n da li ka n o l e h pr ot e i n s uc r o s e t r a n s po r t e r ( S UT ) . S P S m e r upa ka n e n z im y a n g b e r f u n g s i u n t uk m e n gka t a l i s f r ukt o s a 6 ph o s ph a t e da n UD P G l uc o s e m e nj a d i s uc r o s e 6 ph o s ph a t e . S e da n gka n S uc r o s e T r a n s po r t ( S U T ) m e r upa ka n pr ot e in m e m br a n y a n g b e r pe r a n p e n t i n g da l a m a ku mu l a s i s ukr o s a pa da t a n a m a n . T r a n s f o r m a s i pa d a t a n a m a n to m a t de n ga n pe n a m ba ha n ge n S P S a ka n m e ny e b a b k a n pe ni ngka t a n bi o s i n t e s i s s ukr o s a , s e da n gka n pe n a m ba h a n SUT a k a n m e ni ngka t ka n t r a n s l o ka s i s ukr o s a da r i s our c e ke s ink . T r a n s f o r m a s i ge n SP S da n SUT pa da t a n a m a n t o m a t b e r t u j ua n a ga r t a n a m a n to m a t m e n ga l a mi o v e r e k s pr e s i ga n da S P S da n S UT . P a da pe n e li t i a n s e b e l u m n y a t e l a h d i da pa t ka n t a n a m a n da r i h a s i l t r a ns f o r m a s i o v e r e ks pr e s i ge n S P S da n SU T , n a m u n b a ga im a n a pe n ga r u h o v e r e ks pr e s i SoSP S1 da n S oSUT1 da l a m m e ni ng ka t ka n ka n du n g a n pr ot e i n S P S da n S UT , s i n t e s i s d a n t r a n s l o ka s i s ukr o s a pe r l u d i t e l i t i . T u j ua n da r i pe n e li t i a n y a n g d il a kuka n a da l a h u n t uk m e n ge t a h u i ka r a kt e r f i s i o l o g i s t a n a m a n t o m a t t r a n s ge ni k o v e r e ks pr e s i ge n SoSP S1 da n SoSUT1 . P e n e li t i a n i n i d il a k s a n a ka n de n g a n be b e r a pa m e t ode y a i t u: 1) pe na n a m a n t a n a m a n t o m a t tr a n s ge ni k da n ko n t r o l , 2) i s o l a s i t a n a m a n ge n o m , 3) a n a li s i s P C R , 4) e ks t r a ks i pr ot e i n S P S da n S UT , 5) a n a li s i s w e s ter n blot , 6) a n a li s i s ka n du n ga n s ukr o s a . Ha s il pe n e li t i a n m e n u nj ukk a n b a h wa da r i s e m u a t a n a m a n t o m a t put a t if t r a n s ge ni k y a n g d i a n a li s i s t e r ny a t a h a ny a e v e nt 4. 4 y a n g t e r de t e ks i o v e r e ks pr e s i ga n da ge n SoS P S1 da n SoSUT1 . T i da k t e r de t e ks i ny a s e b a g i a n t a na m a n y a n g d i a n a li s i s m e ny e b a b ka n a n a li s i s l e bi h d if o kus k a n pa d a t a n a m a n t o m a t y a ng t e r de t e ks i po s i t i f . P a d a a n a li s i s w e s te r n blot m e m bukt i ka n b a h wa ge n SoSUT 1 m a upu n SoSP S1 y a n g d ii ns e r s i ka n ke da l a m ge n o m t a n a m a n t o m a t da pa t d i e k s pr e s i ka n pa da t i n gk a t tr a n s l a s i s e hi ngga d a pa t m e ni n gk a t ka n ka n du n ga n pr ot e i n S P S m a upu n S UT . S e l a nj ut ny a pa d a a n a li s i s l a i nn y a t e r b ukt i b a h w a de n ga n o v e r e ks pr e s i ga n da ge n SoS P S1 da n SoSUT1 da pa t m e ni ngka t ka n ka n du n ga n s ukr o s a da u n da n b ua h , pe r t u m b u h a n s e r t a pr o duks i t a n a m a na n t o m a t t r a n s ge ni k .en_US
dc.language.isootheren_US
dc.relation.ispartofseries0 8 1510 50 11 49;
dc.subjectT o m a t ( L y c ope r s icon e s c ulentum ) , Suc r os e P hos phate Sy nthas e ( SP S ) , Suc r os e T r an s por te r ( SUT ) , Ov e r e k s pr e s i , B i o s i n t e s is S ukr o s a , T r a n s l o ka s i S ukr o s aen_US
dc.titleKARA KT E RI S A S I F I S I OL OG I S T AN AM AN T O M AT ( L y c ope r s icon e s c u lent u m M il l. ) T RA NSG E NI K OVE RE KS P RE S I GE N S o S P S 1 DAN S o S UT 1en_US
dc.typeOtheren_US


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record