UJI AKTIVITAS ANTIOKSIDAN FRAKSI DARI EKSTRAK ETIL ASETAT BUAH BUNI (Antidesma bunius L.) DI DAERAH JEMBER)
Abstract
Antioxidant activity and identification of antioxidative compounds of
Antidesma bunius (L.) Spreng from Jember were investigated. The fruits was
extracted whit n-hexane and etyl acetat. Extract was concentrated using rotary
evaporator. Etyl acetate Extract was partitioned using column chromatography with
stationary phase Silica gel 60 and mobile phase chloroform : methanol (96 % : 4
%).The process produced tree fractions. DPPH method were used to examine the
antioxidant activity of the etyl acetate extract and the most active fractions (third
fraction). The extract exhibited antioxidant activity in DPPH method with EC50 of
478,486 µg/ml and 466,011 µg/ml for fraction. Chemical analysis was indicated that
the antioxidative compound in the fruits was flavonoid group.
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- UT-Faculty of Pharmacy [1490]