Profil Protein Hati Tikus (Rattus norvegicus) Galur Wistar yang Diinduksi Protein Sintetik Pengkode Protein Spike SARS-CoV-2 Sebagai Poster

Abstract

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) is the virus that causes COVID-19 (Coronavirus Disease 2019). The virus was first identified in late 2019 in Wuhan, China, and has since spread around the world, causing a global pandemic. One of the efforts to prevent the expansion of the spread with the development of targeted vaccine manufacturing, COVID-19 vaccines in general are spike proteins. important research in the development of COVID-19 vaccines successfully created antibodies that can target SARS-CoV-2 spike proteins and show potential as vaccine candidates. This study provides very important insights in the development of a COVID-19 vaccine, one of which has been carried out in the form of research in the production of recombinant proteins encoding the SARS-CoV-2 spike protein of the Indonesian strain. Recombinant protein production is carried out in silico through a bioinformatics approach. The protein was also produced in vitro through GeneticaScience. The effectiveness of the recombinant protein as an antigen has been studied in vivo to determine its effectiveness in inducing immunity and then tested on the liver organ of White Rats (Rattus norvegicus) test animals and then analyzed the concentration levels using the Bradford method. Protein profiling is done by extracting proteins from the organ then tested using the SDS-PAGE method. The results of SDS-PAGE were then analyzed using ImageJ software and then tested using the SDS-PAGE method.