Filogenetik Bakteri Multidrug Resistance pada Penderita Diabetes dan Pemanfaatannya sebagai Book Chapter

Abstract

Some of bacteria that found in diabetic ulcer patients are Multidrug Resistance (MDR). Multidrug Resistance (MDR) is a condition when bacteria are not sensitive to various antibiotics used. Phylogenetic construction is necessary to determine the relationship between MDR bacteria in diabetic ulcer patients. The purpose of this research is to develop new antibiotics or treatments that are more effective and targeted to overcome antibiotic resistance. This research was conducted at the Laboratory of Molecular Medicine CDAST, University of Jember from January to May 2024. The data used in this study came from the 16S rRNA gene sequencing results of MDR bacteria in diabetic ulcer patients isolated by Risqiyah et al. (2022), Narulita et al. (2023) and GenBank NCBI. The results of aligning the 16SrRNA gene sequences of MDR bacteria in patients with diabetic ulcers showed that the sequences of Pseudomonas sp. FP1911 and Proteus mirabilis strain YPM35 had insertions. The sequence of Helicobacter suis NHP19- 4022 had a deletion. The sequence of Proteus mirabilis strain YPM35 had a mutation that caused an amino acid change. The results of genetic distance analysis showed that the species that have the greatest genetic distance is Proteus sp. strain DZ0503SBS3 with Proteus mirabilis strain MPE5203. While the species that has the smallest genetic distance is the species Pseudomonas sp. FP1911 with Corynebacterium sp. strain PY11. The results of the phylogenetic tree reconstruction of MDR bacteria in patients with diabetic ulcers formed 2 large clades. The branch that supports Streptomyces violaceoruber strain S21 with Pseudomonas sp. FP1911 had a high branch consistency value, meaning there will be no change in this branch. Alcaligenes faecalis strain T17 is in a branch with Helicobacter suis NHP19-4022 with a low bootstrap value so the branch of the two species may change.