Pemanfaatan Nanotitanium dalam Sterilisasi dan Regenerasi Tanaman Porang (Amorphophallus Muelleri) Secara In Vitro

Abstract

Porang plant (Amorphophallus muelleri) has the potential to become an export commodity in the industrial and agricultural sectors, which is prospective for development. However, cultivation activities are still constrained by an insufficient supply of porang seeds so production cannot meet export demand. Strategies to increase the production of porang plants can be carried out using conventional methods or vegetative biotechnology, through in vitro culture techniques. Constraints that often occur in the application of in vitro culture often experience contamination. The addition of a sterilizing agent in the form of nanotianium functions as a sterilizing agent without causing tissue damage, besides that it can increase the frequency of plant regeneration and effective bactericidal activity without negative changes. This study aims to determine the effect of the combined application of nanotitanium on explant immersion concentrations and nanotitanium (TiO2) concentrations in the planting medium. The study was conducted using a completely randomized design. The factors studied were the combination of nanotitanium (TiO2) concentration in explant immersion and nanotitanium (TiO2) concentration in the planting medium. The factors studied were the combination of nanotitanium application in explant immersion concentrations and nanotitanium (TiO2) concentrations in the planting medium. The research treatment consisted of 12 treatments which were repeated 3 times. The results obtained showed the best nanotianium combination application with 10 treatments (5'+50 ppm; 10 ppm media) which was characterized by the percentage of 100% sterile explants and produced callus with 67% percentage, 67% roots and 33% shoots with callus appearance time (60 DAP), root emergence time (74 DAP) and shoot emergence time (104 DAP).