| dc.description.abstract | Diazinon is a pesticide widely used by farmers to control
pests. Exposure to the low doses of diazinon can occur
continuously through a polluted environment and
diazinon residues in agricultural products. It has a
nephrotoxic effect through oxidative stress mechanism.
Flavonoid as antioxidant can significantly neutralize
oxidative stress. Shallot peel is a source of flavonoids.
This study was designed to determine the antioxidant
effect of shallot skin infusion (SPI) on kidney oxidative
stress in diazinon-induced Wistar rats by measuring the
flavonoid level of SPI and kidney malondialdehyde
(MDA) level. Rats were divided into normal, diazinon,
and SPI groups. Diazinon was administered at dose of 40
mg/kgBW for 7 days followed by SPI at doses of 500,
1,000, and 2,000 mg/kgBW for 7 days. The flavonoid
level of SPI was measured using spectrophotometry
method and the kidney MDA level was measured using
ELISA method. The flavonoid level of SPI was 96.8 mg
QE/L. The results showed that the normal group had the
lowest kidney MDA level of 2.585 M/mL, the diazinon
group had the highest level of 2.708 M/mL, while the
SPI group at dose of of 500, 1,000, and 2,000 mg/kgBW
had renal MDA levels of 2.642 M/mL, 2.644 M/mL,
and 2.593 M/mL. Nevertheless, the result of statistical
analysis showed that the kidney MDA levels seven days
after diazinon administration was not significantly
different from the normal group so that SPI
administration did not affect the kidney MDA level in
diazinon-induced Wistar rats. | en_US |
