| dc.description.abstract | Exogenous hormones can be used within media to meet the high efficiency on plant
regeneration during somatic embryogenesis. In Indonesian cotton plants, the
efficiency of regeneration media as indicated by the morphological response of
cotton plants and the expression of embryogenesis genes needs to be studied. The
aims of this study were to determine the effect of 2,4-D and IBA hormones on the
morphology and induction of cotton callus; to determine the effect of regeneration
media with the different combination of auxin and cytokinin on the morphology and
gene expression of cotton plant during embryogenesis. This research was
conducted by inducing cotton callus on MS0 medium, MS Basal + 0.1 mg/L 2,4-D
and MS Basal + 0.1 mg/L IBA. The best callus induction results were regenerated
on regeneration media M1 (MS Vitamin + 0.01 ppm 2,4-D + 0.3 ppm IBA + 0.5
ppm Kinetin), M2 (MS Vitamin + 0.01 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm
Kinetin), M3 (MS Vitamin + 0.00 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin),
and M4 (MS Vitamin + 0, 00 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm Kinetin).
Embryogenesis gene expression was observed through RNA isolation, cDNA
synthesis, PCR, and visualization methods. The best morphological results for
callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a
compact and white callus texture character that indicates high embryogenic and
regeneration ability. In the regeneration phase, the highest percentage of
regenerated callus parameters was found in M2 media. Several embryogenesis
genes were expressed such as GhSERK1, GhSERK2, LEC, GhWUS, and
GhWOX11. The best regeneration media with morphological parameters and
embryogenesis gene expression was obtained on M2 media. | en_US |
