• Login
    View Item 
    •   Home
    • MASTER THESES (Koleksi Tesis)
    • MT-Agronomy
    • View Item
    •   Home
    • MASTER THESES (Koleksi Tesis)
    • MT-Agronomy
    • View Item
    JavaScript is disabled for your browser. Some features of this site may not work without it.

    Induksi dan Kultur Suspensi Friable Embryogenic Callus Ubi Kayu (Manihot esculenta) Menggunakan Kultivar Lokal dan Jenis Auksin yang Berbeda

    Thumbnail
    View/Open
    tesis repositroy.pdf (2.615Mb)
    Date
    2023-07-28
    Author
    SIMAMORA, Endah Cahyani
    Metadata
    Show full item record
    Abstract
    Cassava has become one of the prioritized commodities for development by the government and is a relatively easy crop to cultivate. However, there are several challenges in generating embryogenic callus, such as the genotypes of the plants and the exploration of methods and protocols for FEC induction from various cassava cultivars. The research was conducted at the Laboratory of Ecophysiology and Tissue Culture, Faculty of Agriculture, from November 2022 to June 2023. The study involved started from in vitro culture multiplication of cassava plants, callus induction from swollen structures formed by the development of auxiliary buds, followed by FEC induction from several cultivars, namely Kaspro (V1), Ketan (V2), and Kuning (V3) on GD media. This was followed by proliferation in suspension culture with the application of auxin plant growth regulators (PGR), namely picloram at 12 mg/L (P1), 20 mg/L (P2), and 2,4-D at 12 mg/L (P3). The results of the study showed that the callus response of local cultivars resulted in FEC with similar proliferation capabilities, as observed through variables such as suspension volume, fresh weight, and cell viability because of FEC was selected on solid medium before suspension. The level of callus proliferation with different types of auxin showed differences in the growth of FEC suspension based on the increase in fresh weight, with P1 treatment resulting in an average fresh weight of 1.19 grams. The callus response indicated there was interaction between the use of different cultivar types when applied simultaneously with different types of auxin, resulting in the highest fresh weight in the V2P1 treatment, which was 0.64 grams.
    URI
    https://repository.unej.ac.id/xmlui/handle/123456789/119254
    Collections
    • MT-Agronomy [37]

    UPA-TIK Copyright © 2024  Library University of Jember
    Contact Us | Send Feedback

    Indonesia DSpace Group :

    University of Jember Repository
    IPB University Scientific Repository
    UIN Syarif Hidayatullah Institutional Repository
     

     

    Browse

    All of RepositoryCommunities & CollectionsBy Issue DateAuthorsTitlesSubjectsThis CollectionBy Issue DateAuthorsTitlesSubjects

    My Account

    LoginRegister

    UPA-TIK Copyright © 2024  Library University of Jember
    Contact Us | Send Feedback

    Indonesia DSpace Group :

    University of Jember Repository
    IPB University Scientific Repository
    UIN Syarif Hidayatullah Institutional Repository