| dc.description.abstract | This study examines the efficacy of kojic acid and Robusta BP-42 coffee bean extract in
reducing skin hyperpigmentation in rats with skin grafts. Kojic acid is a commonly used
compound for pigmentation reduction; however, it can cause adverse effects like skin
irritation, redness, and itching in some patients. Robusta BP-42 is a superior variety of
robusta coffee found in Indonesia, specifically provided by the Indonesian Coffee and Cocoa
Research Institute in Jember Regency, East Java. BP-42 coffee beans are distinguished by
their exceptional quality and sensory attributes compared to other strains. The experiment
involved three groups of rats with grafted skin: one group was treated with a 2% kojic acid
gel (positive control), another with a 5% robusta BP-42 coffee extract gel, and the third with
a placebo gel (negative control). The grafted skin was observed and photographed on days 0,
4, 7, 11, 18, and 21, with skin pigmentation measurements conducted using Image J software.
Histological analysis was performed on days 7, 14, and 21 for all treatment groups.These
preparations were stained immunohistochemically with anti-tyrosinase antibody (T311). The
results of the immunohistochemical examination in the negative control group showed the
presence of melanocyte cell anti-tyrosinase antibody expression that was immunopositive on
days 7, 14, and 21. Meanwhile, the positive control and treatment groups showed the same
results, decreasing melanocyte cell anti-tyrosinase antibody expression from immunopositive
to immunonegative starting on day 14. This research proves that the gel of robusta coffee
extract administration decreases melanin production in skin graft wound healing. So robusta
coffee extract gel can be an alternative to prevent the process of increasing pigmentation after
skin grafting. | en_US |
