| dc.description.abstract | Background: Periodontitis is an inflammatory disease of the teeth-supporting tissues
caused by microorganisms. Robusta coffee bean extract has antibacterial properties due to its caf feine, flavonoids, trigonelline, and chlorogenic acid contents. The robusta coffee bean extract also
regulates alveolar bone healing through bone remodelling.
Aim: The study aimed to investigate robusta coffee bean extract to inhibit bacterial growth and
accelerate bone repair in vitro and in vivo.
Methods: This study used the paper disc diffusion method with the research group of robusta
coffee bean extract with concentrations of 50%, 25%, 12.5%, 6.25%, and negative control, as much
as 20 and dripped onto the disc paper then placed on the surface of the agar media that had been
inoculated with bacteria. The diameter of the inhibition zone was measured. Twenty periodontitis
rat models were given 0.05 ml of the robusta coffee bean extract on the molars and put in a peri odontal pocket for seven days. Rats were decapitated, and alveolar bone tissues were stained with
HE and IHC staining. The number of osteoclasts, osteoblasts and BMP-2 was counted using a
microscope. Statistical test with Kruskal Wallis followed by Mann Whitney showed a p-value of
<0.05. | en_US |
