Efektivitas Ekstrak Jamu Merek A, B, C, D terhadap Stabilisasi Membran Sel Darah Merah dan Inhibisi Denaturasi Protein

Abstract

Non-steroidal anti-inflammatory drugs (NSAIDs) are potent anti-inflammatory agents with gastrointestinal, cardiovascular, and renal side effects. Therefore other anti-inflammatory agents with minimal side effects are still needed. Since ancient times, Indonesian people have used herbal medicine to treat various health problems. The existence of modernization has led to an increasing trend of using instant jamu from e-commerce, including jamu brands A, B, C, D. One of the obstacles in the development of jamu in the health sector is the lack of evidence based. Aim: to evaluate the anti-inflammatory activity of A, B, C, D jamu through its IC50 value and to provide evidence based on A, B, C, D brands jamu as anti-inflammatory agents. Research methodology: in vitro anti-inflammatory activity test using stabilization of rabbit red blood cell (RBC) membranes and inhibition of protein Bovine Serum Albumin (BSA) denaturation. Results: The IC50 value of diclofenac sodium in the red blood cell membrane stabilization method was 104.671 ± 3.803 µg/mL. Whereas in the protein denaturation inhibition method was 4.699 ± 0.325 µg/mL. Respectively the IC50 values of jamu brands A, B, C, D on the red blood cell membrane stabilization method were 959,231 ± 49,861; 619,367 ± 4,197; 33,753,238 ± 1,940,251; 15,732,179 ± 798,464 µg/mL. Meanwhile, the IC50 value of jamu in the protein denaturation method was 98.627 ± 2.423; 73.629 ± 2.069; 1,793,989 ± 9,177; 897.431 ± 5.622 µg/mL. These results statistically different (p<0.01). Conclusion: jamu brands A, B, C, D are effective in stabilizing red blood cell membranes and inhibiting protein denaturation with various IC50 values. Herbal anti-inflammatory activity B > A > D > C in both test methods.