| dc.description.abstract | Shigella dysenteriae is an obligate pathogen that usually found in clinical specimen
from shigelosis patients and causes gastrointestinal tract infections with high
morbidity and mortality. The pathogenic mechanism of the bacteria is not fully
elucidated especially its potential activity of the pili as hemaglutinin and adhesion
molecule. The aim of this study is to predict the molecule weight of pili and OMP
from S. dysenteriae. The research stages included identification of S. dysenteriae
isolation of OMP S. dysenteriae, sodium dodecyl sulphate–polyacrylamide gel
electrophoresis (SDS-PAGE), hemagglutination test, isolation of enterocyte of
BALB/c mice and and adhesion test. The study showed that the molecule weight
protein of S. dysenteriae were 155 kDa, 124 kDa, 95 kDa, 78 kDa, and 32 kDa. The
protein pili S. dysenteriae with a molecular weight of 95 kDa functions as an adhesin.
Changes in pili protein concentration of 95 kDa molecular weight S. dysenteriae
coated on the enterocyte of mice Balb/c strain, had a significant effect on the
adhesion index. The protein pili S. dysenteriae with a molecular weight of 95 kDa
functions as an adhesin. | en_US |
