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dc.contributor.authorRIYANTI, Rini
dc.date.accessioned2023-05-18T13:10:47Z
dc.date.available2023-05-18T13:10:47Z
dc.date.issued2019
dc.identifier.urihttps://repository.unej.ac.id/xmlui/handle/123456789/116229
dc.description.abstractThe main pathophysiology basis of E-thalassemia is excess unbound D-globin chain due to reduction D-globin expression. Reduction of D-globin chain can lead clinical improvement. Reduction of D-globin chain is an alternative therapy in E major thalassemia patients. microRNA (miRNA) can regulate D-globin chain through targeting transcription factor. Klfd and GATA-1 erythroid transcription factor regulated by miRNA-144. This study was an experimental study using PBMC of major E thalassemia patient. PBMC divided into two groups that were not transfected and transfected anti-miRNA 144. The expression of miRNA-144 were detected using Exiqon's miRCURY LNATM universal RT microRNA PCR. The expression of D-globin chain protein were detected using immunobloting technique.There are differences in the expression of miRNA-144 who have carried out anti-miRNA 144 transfections with those that have not been conducted transfections in PBMC major E thalassemia patients. There are no differences in the expression of D globin chain who have carried out anti-miRNA 144 transfections with those that have not been conducted transfections in PBMC major E thalassemia patients. Based on this study, the administration of anti-miRNA 144 cannot decrease the expression D globin chain expression in PBMC major E thalassemia patients.en_US
dc.language.isoenen_US
dc.publisherIndian of Journal Clinical Biochemistryen_US
dc.subjectmiRNA 144en_US
dc.subjectGlobin Chainen_US
dc.subjectPBMCen_US
dc.subjectPeripheral Blood Mononuclear cellen_US
dc.subjectMajor Beta Thalassemia Patientsen_US
dc.titleThe Effect of anti miRNA 144 on the Expression of a Globin Chain in PBMC (Peripheral Blood Mononuclear cell) of Major Beta Thalassemia Patientsen_US
dc.typeArticleen_US


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