| dc.description.abstract | Investigating the function of combining induced rat monocytes-derived bone marrow-haemopoietic
stem cell (rat BM-HSCs) with LPS and rat bone marrow-mesenchymal stem cell (rat BM-MSCs) was
to analyze the acceleration of homing process mechanism in injured pancreas. Mononucleated
stem cells were isolated from aspirated whole rat BM using ficoll and cultured in α-MEM complete
growth medium in 10 cm petridish. After two days, adherent cells after washing twice in petridish
were added α-MEM growth medium and then mesenchymal cells were characterized using CD105
marker in third passage and labeled PKH26. Then haemopoietic stem cells (HSCs) were isolated
with magnetic beads CD34+ and differentiated in vitro, and then induced monocytes with LPS.
Animal experiment used 28 male Wistar rats, and divided them into 4 groups. After transplantation combined, both cells between monocyte derived HSc (mHSCs) and rat BM-MSC were analyzed
expression of pair box gen 4 (Pax4), pancreatic and duodenal homeobox (Pdx1), C-peptide using
immunohistochemistry, then secretion of insulin and C-peptide analyzed using in-direct ELISA.
Results showed that the expressions of Pax4, Pdx1, C-peptide found in the surface membrane cell
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of pancreatic cell, and secreted C-peptide and insulin were shown significant (P < 0.05) in transplanted group 2, 3 and 4, but in group 3 were transplanted with combined cells more dominant
than non-combined cells. Conclusions suggested that combining of induced monocytes-derived
HSCs and rat BM-MSCs has accelerated homing MSCs into injured pancreatic tissue. | en_US |
