| dc.description.abstract | BACKGROUND
Dengue virus is transmitted by several species of Aedes mosquitoes, with
Aedes albopictus assecondary vector. During blood feeding, these vectors
inject saliva into the vertebrate hosts. The saliva contains anticoagulant,
anti-inflammatory and immunogenic factors. The objective of this research
was to detect immunogenic proteins from Ae.albopictus salivary glands
reacting with sera of people living in dengue endemic areas.
METHODS
The identification of immunogenic proteins of Ae. albopictus salivary gland
used one-dimensional gel electrophoresis (sodium dodecyl sulfate
polyacrylamide gel electrophoresis), and western blot analysis, respectively.
To determine the immunogenic nature of the candidate proteins, the
antigens from the salivary gland of Ae. albopictus were reacted with sera
from healthy persons, dengue hemorrhagic fever (DHF) patients, and
neonates, each of the groups comprising 10 samples.
RESULTS
The protein profiles of Ae. albopictus salivary glands showed 13 bands
with molecular weights from 16 kDa up to 97 kDa, i.e. 16, 17, 26, 28, 31, 32,
45, 55, 60, 67, 73, 76, and 97 kDa. According to western blot analysis result,
the 31 kDa proteins were recognized in all endemic population sera, both in
DHF patients and healthy persons. In contrast, protein bands of 47 and 67
kDa were only recognized by the sera of DHF patients.
CONCLUSION
Three immunogenic proteins of 31, 47 and 67 kDa were detected from Ae.
albopictus salivary glands. These immunogenic proteins may be developed
as candidate biomarkers for bite exposure to Ae. albopictus and as vectorbased DHF vaccines. | en_US |
