| dc.description.abstract | Malaria is a public health threat caused by the Plasmodium infection
transmitted by the Anopheles mosquito. Malaria vector control is highly
dependent on the ability to determine mosquito species' vectorial and bionomic
capacity. Species identification based on morphological characteristics as well
as DNA-barcoding approaches is a very important step in determining
vectorial capacity. Our research group has redesigned a new ITS2 primer,
namely, sma-its2, which is specifically able to identify the Anopheles (An.)
mosquito vector but cannot identify other mosquito vectors. This study wanted
to test this primer's specificity further for identification of other Anopheles
mosquitoes. We used An. minimus collected from Kulonprogo, Yogyakarta –
Indonesia. The methods used in this research are as follows: landing collection,
morphological identification, isolation of genomic DNA, PCR (Polymerase
Chain Reaction), PCR product purification, sequencing, and data analysis. An.
vagus from Bangsring, Banyuwangi – Indonesia, which had previously been
identified using the same primary, was used as a positive control. The results
of the morphological analysis showed that both species were in accordance
with the vector identification key used in this study. The molecular analysis
showed that the sma-its2 primer could amplify the ITS2 sequence of An. vagus
and An. minimus, producing 650 – 700 bp. However, further analysis of the
ITS2 sequences of both species, resulted in the same species, namely An.
vagus, with a different accession number in GenBank. This showed that the
sma-its2 primar can be used to identify An. vagus but cannot be used to identify
An. minimus. Analysis of the primer position in the ITS2 sequences showed
the presence of 3 nucleotides in the forward sma-its2 primer that was not
recognized by the An. minimus sequences and thus, hinder the successful
identification of these species. | en_US |
