Examination of Coffee Pulp Waste for Medium in Cellulase Production by Aspergillus Species

Abstract

An isolate, identified as Aspergillus species can grow well by introducing coffee pulp medium as carbon and nitrogen source in solid state fermentation without any nutrient added. This genus optimum produced cellulase in five days of fermentation at 37°C. Purification, gave the yield 0.13% and 477 fold. The enzyme stable at pH 3.5 to 6.5 and below 55°C, while the optimum activity at pH 5 and 50°C respectively. It is suggested that coffee pulp waste can be used as a cheap medium for cellulase production.