| dc.description.abstract | The human body needs an antioxidant-rich diet
that comes from foods, beverages, and herbal products to
support the physiological antioxidant systems. Thus, the
development of an analytical tool for a simple assay of the
total antioxidant capacity (TAC) of the rich antioxidant
samples is crucial. The current work demonstrates a simple
colorimetric assay of TAC of the herbal extract on the
paper microzone plate (PlZP) that was constructed in the
70-well of patterned paper using the screen printing technique. The PlZP was constructed by immobilizing DPPH
(2,2-diphenyl-1-picrylhydrazyl) onto 70-well of PlZP as a
sensing zone for colorimetric detection. The purple-sensing
zone exhibited a good response to gallic acid (GA), by
producing slightly gray to pale yellow color that can be
captured using a scanner and then analyzed using the
ImageJ program. The paper-based sensor showed a linear
response toward GA at 0.05–0.6 mM (r = 0.9895), reproducible response (RSD \ 4%), and accurate measurement
with 91 to 106% recovery for measuring TAC of herbal
extract, presented as mM gallic acid equivalent and showed
a good agreement with the standard DPPH method. The
results suggested that the proposed method can be applied
for simple TAC measurement in the herbal extract. | en_US |
