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dc.contributor.authorKUSWANDI, Bambang
dc.contributor.authorFANTONI, Muhammad
dc.contributor.authorHIDAYAT, Mochammad Amrun
dc.contributor.authorNINGSIH, Indah Yulia
dc.date.accessioned2022-04-22T03:53:27Z
dc.date.available2022-04-22T03:53:27Z
dc.date.issued2020-04-03
dc.identifier.govdocKODEPRODI2210101#Farmasi
dc.identifier.govdocNIDN 0001026907
dc.identifier.govdocNIDN0026017801
dc.identifier.govdocNIDN0012078401
dc.identifier.urihttp://repository.unej.ac.id/xmlui/handle/123456789/106552
dc.description.abstractThe human body needs an antioxidant-rich diet that comes from foods, beverages, and herbal products to support the physiological antioxidant systems. Thus, the development of an analytical tool for a simple assay of the total antioxidant capacity (TAC) of the rich antioxidant samples is crucial. The current work demonstrates a simple colorimetric assay of TAC of the herbal extract on the paper microzone plate (PlZP) that was constructed in the 70-well of patterned paper using the screen printing technique. The PlZP was constructed by immobilizing DPPH (2,2-diphenyl-1-picrylhydrazyl) onto 70-well of PlZP as a sensing zone for colorimetric detection. The purple-sensing zone exhibited a good response to gallic acid (GA), by producing slightly gray to pale yellow color that can be captured using a scanner and then analyzed using the ImageJ program. The paper-based sensor showed a linear response toward GA at 0.05–0.6 mM (r = 0.9895), reproducible response (RSD \ 4%), and accurate measurement with 91 to 106% recovery for measuring TAC of herbal extract, presented as mM gallic acid equivalent and showed a good agreement with the standard DPPH method. The results suggested that the proposed method can be applied for simple TAC measurement in the herbal extract.en_US
dc.language.isoenen_US
dc.publisherJ Food Sci Technolen_US
dc.subjectPaper microzone plateen_US
dc.subjectPaper-based sensoren_US
dc.subjectColorimetricen_US
dc.subjectDPPHen_US
dc.subjectTotal antioxidant capacityen_US
dc.subjectHerbal extracten_US
dc.titlePaper microzone plate based on DPPH as a simple colorimetric assay of the total antioxidant content of herbal extractsen_US
dc.typeArticleen_US


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