Sugar-Rich Hydrolyzates from Coffee Pulp Waste which Produced under Solid State Fermentation by Pestalotiosis sp. VM9 and Aspergillus sp. VTM5, and Its Efficiency as Medium for Single Cell Protein Saccharomyces cerevisiae

Abstract

Polysaccharides rich coffee pulp is one of some abundant agricultural waste in Indonesia. The pulp also contains protein which may lead to be an important source for industrial bioprocessing. Under solid-state fermentation (SSF) by Pestalotiosis sp VM9 using of this pulp, reducing sugar was produced 392.35 µg/ml after 6 days incubation at 30o C. Meanwhile, SSF of the pulp by Aspergillus sp. VTM5, after 5 days of incubation 446.6 µg/ml of reducing was released. The investigation proved that the hydrolyzates medium which were produced under SSF by Pestalotiosis sp VM9 and Aspergillus sp. VTM5 can be used as a source for single cell protein (SCP) Saccharomyces cerevisiae production. The SCP productions using both hydrolyzates were1.89x106 and 2.9x106 cell/mL after 54 and 48 hours of incubation at 30o C. Furthermore, during SCP production, S. Cerevisiae consumed sugars as carbon sources in a range of 189.8- 225.5 µg/ml (49.5-51.6%) from initial concentration. From the results, it is suggested that the coffee pulp waste can be used as a cheap medium for SCP production. Further investigation to improve SCP production efficiency, optimizing of hydrolysis under SSF and analysis of hydrolyzates component were needed.