| dc.description.abstract | Coffee pulp biomass waste can easily be found anywhere in Indonesia, considering it is
the fourth world's largest coffee exporter. The utilization of coffee pulp is very limited and is
categorized as a source of pollutants in water bodies and soils. In contrast, coffee pulp waste is very
potential because 63% of the main compound is cellulose. Microbial utilization of this waste for
enzyme production purposes, especially cellulase, is a breakthrough that may lead to reduce
production costs. Initial investigations showed that Aspergillus sp. VTM1 through solid-state
fermentation (SSF) could produce cellulases. Optimal cellulase could be produced if 10 g coffee
pulp with 10% moisture is inoculated using 108
spores/mL of Aspergillus sp. VTM1 for 48 hours at
30 °C. Hydrolysis of 1% carboxymethyl cellulose (CMC) substrate in 50 mM acetate buffer pH 5
by this cellulase showed that the enzyme activity reached up to 1.18 U/mL. The optimum pH of the
enzyme was 5 and stable at 3-3.5 and 4-7. The success of the first step of this investigation will be a
cheap way of producing cellulases. | en_US |
