| dc.description.abstract | About thirty-five percent of coffee pulp waste is pectin. It may potentially be a source to
be used in the bioprocessing industry. For example, it can be used as a substrate to produce
pectinase from microorganisms under solid-state fermentation (SSF). In this investigation, an
isolated fungus VTM4 with density 107 spores/mL was grown on coffee pulp medium-based, and
after 0-168 hours incubation at 30 °C, pectinase activity was detected. The activity was measured
based on reducing sugar released by crude pectinase against 0.5% alkali extract pectin substrate in
20 mM buffer acetate pH 5. The highest reducing sugar produced was 223.34 µg/mL after 72 hours
of incubation at 30 °C. The optimum pH on enzyme activity was 4 with the maximum activity
0.747 U/mL and was stable (more than 80%) at a pH range of 4-5.5. The results revealed that the
coffee substrate could be utilized as a carbon and nitrogen source to produce pectinase. Further
research on purification and characterization of the enzyme to improve pectinase yield production
was needed. | en_US |
