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dc.contributor.authorGASANI, Okta Novalia
dc.contributor.authorA.AZIZAH, A. Azizah
dc.contributor.authorSISWANTO, Siswanto
dc.contributor.authorWINARSA, Rudju
dc.date.accessioned2022-04-11T04:30:19Z
dc.date.available2022-04-11T04:30:19Z
dc.date.issued2021-05-04
dc.identifier.govdocKODEPRODI1810401#Biologi
dc.identifier.govdocNIDN0003056808
dc.identifier.urihttp://repository.unej.ac.id/xmlui/handle/123456789/106319
dc.description.abstractAbout thirty-five percent of coffee pulp waste is pectin. It may potentially be a source to be used in the bioprocessing industry. For example, it can be used as a substrate to produce pectinase from microorganisms under solid-state fermentation (SSF). In this investigation, an isolated fungus VTM4 with density 107 spores/mL was grown on coffee pulp medium-based, and after 0-168 hours incubation at 30 °C, pectinase activity was detected. The activity was measured based on reducing sugar released by crude pectinase against 0.5% alkali extract pectin substrate in 20 mM buffer acetate pH 5. The highest reducing sugar produced was 223.34 µg/mL after 72 hours of incubation at 30 °C. The optimum pH on enzyme activity was 4 with the maximum activity 0.747 U/mL and was stable (more than 80%) at a pH range of 4-5.5. The results revealed that the coffee substrate could be utilized as a carbon and nitrogen source to produce pectinase. Further research on purification and characterization of the enzyme to improve pectinase yield production was needed.en_US
dc.language.isoenen_US
dc.publisherKey Engineering Materialsen_US
dc.subjectcoffee pulpen_US
dc.subjectoptimization of pHen_US
dc.subjectpectinaseen_US
dc.subjectpH stabilityen_US
dc.subjectsolid-state fermentationen_US
dc.titlePectinase Production by Using Coffee Pulp Substrate as Carbon and Nitrogen Sourceen_US
dc.typeArticleen_US


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