Sugar Production by Digesting of Oil Palm Empty Fruit Bunch Using Extracellular Enzymes from Aspergillus niger and Trichoderma reesei for Ethanol Production

Abstract

Extracellular enzymes which obtained from 4 days cultivation Aspergillus niger and Trichoderma reesei on solid state fermentation of oil palm empty fruit bunch (OPEFB) were used for lignocellulosic-rich OPEFB digestion. The enzymes were concentrated using 70% saturated ammonium sulphate, dialysed against 20mM acetate buffer at pH 5 and adjusted one tenth (v/v) from the initial volume with the same buffer. The concentrated enzymes were then used in hydrolysation of powdered OPEFB. Amount of 10.65 mg/ ml and 11.47 mg/ml sugars were produced when each concetrated enzyme A. niger and T. reesei mixed with2%OPEFB. These hydrolysation were done on 100 ml total volume, incubated at 37oC with 100 rpm shaken for 36 hours. Further, both hydrolyzates results were sterilised and fermented anaerobically using Saccharomycess cerevisiae at concentration 0.5mg/ml cells and incubated in 30oC for 24 hours. Colorimetric analysis using QuantiChrom Kit DIET-500 at OD 580nm gave results the alcohol production were 0.86% and 0.92% which were similar with Gas Chromatograph analysis that of 0.83% and 0.93%, respectively.