| dc.description.abstract | Jumbo phage can isolated by two different methods and protocols. It depends on
size, phage species, substrate sources, environment, media and tools. Each method has positive
and negative results within each factors. The research aimed to determine the suitable protocol
to isolate the jumbo phages from winter grass soil during winter conditions, utilizing two main
protocols, the enrichment protocol and non-enrichment protocol. In Sequent, research was
conducted with several steps, including bacterial isolation, jumbo phage isolation, plaque test
in Escherichia coli colony, bacterial genus identification, phage test in soil bacteria, protocol
evaluation, DNA extraction and electrophoresis. Based on the plaque size and plaque total,
each protocol did not significantly differ on plaque total among the E. coli or each isolated soil
bacteria. On the E. coli test, the plaque total average was 65 plaque, nor 45 plaque emerge on
each isolated soil bacteria. The significant difference on each protocol was on the clarity of
plaque. Based on visual observation, the plaques with enrichment protocol were clearer. It was
obvious that the isolation of jumbo phage from the winter grass during winter conditions can
be done without enrichment protocol. It can be isolated with standard isolation protocol | en_US |
